LinKine AP Labeling Kit

Alkaline phosphatase (ALP or AKP) is an enzyme that can dephosphorylate the corresponding substrate by hydrolyzing the phosphate monoester to remove the phosphate group on the substrate molecule and generate phosphate ions and free hydroxyl groups. Such substrates include nucleic acids, proteins, alkaloids and the like. The process of removing the phosphoric acid group is called dephosphorylation or dephosphorylation. Alkaline phosphatase is a kind of phosphatase, but it is not a single enzyme, but a group of isoenzymes. Alkaline phosphatase can be combined with the molecule to be labeled. When incubated with an appropriate substrate, it will generate labeled molecule conjugates with colored, fluorescent or luminescent derivatives, which can be detected and quantitatively analyzed. AP is usually used in conjugates to determine the presence of molecular targets, and is often used in experiments such as ELISA, Western blotting, and immunohistochemistry. Since higher concentrations of inorganic phosphorus can competitively inhibit the activity of alkaline phosphatase, systems such as phosphate-rich PBS should not be used in the immune experiment, otherwise it will cause a high background, and TBS and other non-phosphate-free systems can be used.